We use DNA synthesizer to synthesise oligonucleotides by adopting the solid-phase phosphoramidite method. This is currently the mature and mainstream oligonucleotide synthesis method. We are capable of synthesising oligonucleotides using not only the basic building blocks, such as deoxynucleosides and ribonucleosides, but also chemically modified nucleosides, such as florescence labelled deoxynucleosides.
A purification process is required to obtain high-purity end-product. Purification is the key to the quality of oligonucleotide synthesis. Techniques we currently use for oligonucleotides purification include desalting purification, PAGE purification, affinity purification, and HPLC purification. Oligonucleotides are widely used in most life sciences laboratories and it is well-suited for various applications in molecular biology and medical research. For example, oligonucleotides are used as primers in both DNA sequencing and DNA amplification. Oligonucleotides also serve as probes in various DNA and RNA detection and analysis. Oligonucleotide synthesis products are needed essentially by almost every laboratory in life sciences research.
As a well-recognised provider of customised oligonucleotides in China, we have 22 years of experience in perfecting the production of oligonucleotides. We have a capacity of producing over 10,000 oligonucleotides (about 200,000 bases) per day, adopting different types of purification methods. We are also able to successfully synthesise complex and rare oligonucleotides. A stringent quality assurance system is implemented to ensure the process is carried out in a quality-controlled environment. Please see the section headed “Business — Quality Assurance” for details of our quality control policies and procedures.